Details

Autor(en) / Beteiligte

• Choi, Daheui
• Gonzalez‐Suarez, Alan M.
• Dumbrava, Mihai G.
• Medlyn, Michael
• Hoyos‐Vega, Jose M.
• Cichocki, Frank
• Miller, Jeffrey S.
• Ding, Li
• Zhu, Mojun
• Stybayeva, Gulnaz
• Gaspar‐Maia, Alexandre
• Billadeau, Daniel D.
• Ma, Wen Wee
• Revzin, Alexander

Titel

Microfluidic Organoid Cultures Derived from Pancreatic Cancer Biopsies for Personalized Testing of Chemotherapy and Immunotherapy

Ist Teil von

• Advanced science, 2024-02, Vol.11 (5), p.e2303088-n/a

Ort / Verlag

Germany: John Wiley & Sons, Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Access via Wiley Online Library

Beschreibungen/Notizen

• Patient‐derived cancer organoids (PDOs) hold considerable promise for personalizing therapy selection and improving patient outcomes. However, it is challenging to generate PDOs in sufficient numbers to test therapies in standard culture platforms. This challenge is particularly acute for pancreatic ductal adenocarcinoma (PDAC) where most patients are diagnosed at an advanced stage with non‐resectable tumors and where patient tissue is in the form of needle biopsies. Here the development and characterization of microfluidic devices for testing therapies using a limited amount of tissue or PDOs available from PDAC biopsies is described. It is demonstrated that microfluidic PDOs are phenotypically and genotypically similar to the gold‐standard Matrigel organoids with the advantages of 1) spheroid uniformity, 2) minimal cell number requirement, and 3) not relying on Matrigel. The utility of microfluidic PDOs is proven by testing PDO responses to several chemotherapies, including an inhibitor of glycogen synthase kinase (GSKI). In addition, microfluidic organoid cultures are used to test effectiveness of immunotherapy comprised of NK cells in combination with a novel biologic. In summary, our microfluidic device offers considerable benefits for personalizing oncology based on cancer biopsies and may, in the future, be developed into a companion diagnostic for chemotherapy or immunotherapy treatments. Treatment of pancreatic cancer may be made more effective by selecting therapies for an individual patient. The authors developed and characterized microfluidic devices that may be used to test chemotherapies and immunotherapies using needle core biopsies from patients with pancreatic cancer.