Details

Autor(en) / Beteiligte

• Sigmund, Felix
• Massner, Christoph
• Erdmann, Philipp
• Stelzl, Anja
• Rolbieski, Hannes
• Desai, Mitul
• Bricault, Sarah
• Wörner, Tobias P.
• Snijder, Joost
• Geerlof, Arie
• Fuchs, Helmut
• Hrabĕ de Angelis, Martin
• Heck, Albert J. R.
• Jasanoff, Alan
• Ntziachristos, Vasilis
• Plitzko, Jürgen
• Westmeyer, Gil G.

Titel

Bacterial encapsulins as orthogonal compartments for mammalian cell engineering

Ist Teil von

• Nature communications, 2018-05, Vol.9 (1), p.1990-14, Article 1990

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions and size-constrained metal biomineralization. The shell protein ( EncA ) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian cells to which sets of native ( EncB,C,D ) and engineered cargo proteins self-target enabling localized bimolecular fluorescence and enzyme complementation. Encapsulation of the enzyme tyrosinase leads to the confinement of toxic melanin production for robust detection via multispectral optoacoustic tomography (MSOT). Co-expression of ferritin-like native cargo ( EncB , C ) results in efficient iron sequestration producing substantial contrast by magnetic resonance imaging (MRI) and allowing for magnetic cell sorting. The monodisperse, spherical, and iron-loading nanoshells are also excellent genetically encoded reporters for electron microscopy (EM). In general, eukaryotically expressed encapsulins enable cellular engineering of spatially confined multicomponent processes with versatile applications in multiscale molecular imaging, as well as intriguing implications for metabolic engineering and cellular therapy. Artificial compartments have been expressed in prokaryotes and yeast, but similar capabilities have been missing for mammalian cell engineering. Here the authors use bacterial encapsulins to engineer genetically controlled multifunctional orthogonal compartments in mammalian cells.