Details

Autor(en) / Beteiligte

• Ferreira, Silvia A.
• Motwani, Meghna S.
• Faull, Peter A.
• Seymour, Alexis J.
• Yu, Tracy T. L.
• Enayati, Marjan
• Taheem, Dheraj K.
• Salzlechner, Christoph
• Haghighi, Tabasom
• Kania, Ewa M.
• Oommen, Oommen P.
• Ahmed, Tarek
• Loaiza, Sandra
• Parzych, Katarzyna
• Dazzi, Francesco
• Varghese, Oommen P.
• Festy, Frederic
• Grigoriadis, Agamemnon E.
• Auner, Holger W.
• Snijders, Ambrosius P.
• Bozec, Laurent
• Gentleman, Eileen

Titel

Bi-directional cell-pericellular matrix interactions direct stem cell fate

Ist Teil von

• Nature communications, 2018-10, Vol.9 (1), p.4049-12, Article 4049

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Modifiable hydrogels have revealed tremendous insight into how physical characteristics of cells’ 3D environment drive stem cell lineage specification. However, in native tissues, cells do not passively receive signals from their niche. Instead they actively probe and modify their pericellular space to suit their needs, yet the dynamics of cells’ reciprocal interactions with their pericellular environment when encapsulated within hydrogels remains relatively unexplored. Here, we show that human bone marrow stromal cells (hMSC) encapsulated within hyaluronic acid-based hydrogels modify their surroundings by synthesizing, secreting and arranging proteins pericellularly or by degrading the hydrogel. hMSC’s interactions with this local environment have a role in regulating hMSC fate, with a secreted proteinaceous pericellular matrix associated with adipogenesis, and degradation with osteogenesis. Our observations suggest that hMSC participate in a bi-directional interplay between the properties of their 3D milieu and their own secreted pericellular matrix, and that this combination of interactions drives fate. 3D hydrogels have provided information on the physical requirements of stem cell fate, but the contribution of interactions with the pericellular environment are under-explored. Here the authors show that pericellular matrix secreted by human bone marrow stromal cells (hMSC) embedded in a HA-based hydrogel contribute to hMSC fate.