Details

Autor(en) / Beteiligte

• Gonzalez-Cordero, Anai
• Kruczek, Kamil
• Naeem, Arifa
• Fernando, Milan
• Kloc, Magdalena
• Ribeiro, Joana
• Goh, Debbie
• Duran, Yanai
• Blackford, Samuel J.I.
• Abelleira-Hervas, Laura
• Sampson, Robert D.
• Shum, Ian O.
• Branch, Matthew J.
• Gardner, Peter J.
• Sowden, Jane C.
• Bainbridge, James W.B.
• Smith, Alexander J.
• West, Emma L.
• Pearson, Rachael A.
• Ali, Robin R.

Titel

Recapitulation of Human Retinal Development from Human Pluripotent Stem Cells Generates Transplantable Populations of Cone Photoreceptors

Ist Teil von

• Stem cell reports, 2017-09, Vol.9 (3), p.820-837

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Transplantation of rod photoreceptors, derived either from neonatal retinae or pluripotent stem cells (PSCs), can restore rod-mediated visual function in murine models of inherited blindness. However, humans depend more upon cone photoreceptors that are required for daylight, color, and high-acuity vision. Indeed, macular retinopathies involving loss of cones are leading causes of blindness. An essential step for developing stem cell-based therapies for maculopathies is the ability to generate transplantable human cones from renewable sources. Here, we report a modified 2D/3D protocol for generating hPSC-derived neural retinal vesicles with well-formed ONL-like structures containing cones and rods bearing inner segments and connecting cilia, nascent outer segments, and presynaptic structures. This differentiation system recapitulates human photoreceptor development, allowing the isolation and transplantation of a pure population of stage-matched cones. Purified human long/medium cones survive and become incorporated within the adult mouse retina, supporting the potential of photoreceptor transplantation for treating retinal degeneration. [Display omitted] •hPSC-derived photoreceptors express markers in a pattern similar to human development•2D/3D differentiation protocol generates sufficient cones for transplantation•hPSC-derived cones incorporate into the adult retina following transplantation Gonzalez-Cordero et al. describe a protocol for the robust generation of photoreceptors from human pluripotent stem cells and report the transplantation of a pure population of human cone photoreceptors and their incorporation into the recipient adult murine neural retina.