Membranes (Basel), 2023-04, Vol.13 (5), p.471
2023
Details
- Autor(en) / Beteiligte
- Titel
- Lipoprotein Particles as Shuttles for Hydrophilic Cargo
- Ist Teil von
- Membranes (Basel), 2023-04, Vol.13 (5), p.471
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2023
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Lipoprotein particles (LPs) are excellent transporters and have been intensively studied in cardiovascular diseases, especially regarding parameters such as their class distribution and accumulation, site-specific delivery, cellular internalization, and escape from endo/lysosomal compartments. The aim of the present work is the hydrophilic cargo loading of LPs. As an exemplary proof-of-principle showcase, the glucose metabolism-regulating hormone, insulin, was successfully incorporated into high-density lipoprotein (HDL) particles. The incorporation was studied and verified to be successful using Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM). Single-molecule-sensitive FM together with confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2077-0375eISSN: 2077-0375DOI: 10.3390/membranes13050471Titel-ID: cdi_doaj_primary_oai_doaj_org_article_89b2d2a13be146f885800fbd09079ef9
- Format
- –
- Schlagworte
- Antibodies, Atomic force microscopy, Blood & organ donations, Cardiovascular diseases, Cholesterol, Fluorescence, Fluorescence microscopy, Glucose, Glucose metabolism, Glucose transporter, High density lipoprotein, Hydrophilicity, Insulin, Internalization, Lipids, lipoprotein particles, Lipoproteins, Medical research, Medicin och hälsovetenskap, Metabolism, Microscopy, Penicillin, Physiological aspects, Proteins, shuttle for hydrophilic cargo, Silicon nitride, single-molecule-sensitive fluorescence microscopy, Spectrum analysis, Translocation