G3 : genes - genomes - genetics, 2017-02, Vol.7 (2), p.719-722
2017
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Details
- Autor(en) / Beteiligte
- Titel
- Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection
- Ist Teil von
- G3 : genes - genomes - genetics, 2017-02, Vol.7 (2), p.719-722
- Ort / Verlag
- England: Genetics Society of America
- Erscheinungsjahr
- 2017
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Cpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet, or even surpass, Cas9 targeting rates (approaching 100% targeting), but require higher concentrations of mRNA and guide. We also demonstrate that coinjecting two guides with close targeting sites can result in synergistic genomic cutting, even if one of the guides has minimal cutting activity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2160-1836eISSN: 2160-1836DOI: 10.1534/g3.116.038091Titel-ID: cdi_doaj_primary_oai_doaj_org_article_89683c74cd5d49d182e18109a69beb4d
- Format
- –
- Schlagworte
- Acidaminococcus - enzymology, Acidaminococcus - genetics, Animals, Bacterial Proteins - genetics, Cpf1 nuclease, CRISPR, CRISPR-Cas Systems - genetics, Endonucleases - genetics, Gene Editing - methods, Gene Targeting - methods, genome editing, Investigations, Mice, mouse, RNA, Guide, CRISPR-Cas Systems - genetics, RNA, Messenger - genetics