IET nanobiotechnology, 2019-10, Vol.13 (8), p.875-879
2019
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- Autor(en) / Beteiligte
- Titel
- Photo-inactivation of bacteria in hospital effluent via thiolated iron-doped nanoceria
- Ist Teil von
- IET nanobiotechnology, 2019-10, Vol.13 (8), p.875-879
- Ort / Verlag
- United States: The Institution of Engineering and Technology
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Hospital wastewater is a major contributor of disease-causing microbes and the emergence of antibiotic resistant bacteria. In this study, thiolated iron-doped nanoceria was synthesised and tested for killing of microbes from hospital effluent. These particles were designed to inhibit the efflux pumps of the bacteria found in hospital effluent with further ability to activate in visible light via iron doping thus generating tunable amount of reactive oxygen species (ROS). The quantum yield of the ROS generated by the nanoceria was 0.67 while the ROS types produced were singlet oxygen (36%), hydroxyl radical (31%) and hydroxyl ions (32%), respectively. The particles were initially synthesised through green route using Foeniculum vulgare seeds extract and were annealed at 200°C and further coated with thiolated chitosan to enhance the solubility and efflux pump inhibition. X-ray diffraction confirmed the polycrystalline nature of nanoparticles and uniform spherical shape with 30 nm size, confirmed by scanning electron microscope. The nanoparticles exhibited 100% bactericidal activity at 100 µg/mL against all the isolated bacteria. The enhanced bactericidal effect of iron-doped nanoceria could be attributed to efflux inhibition via thiolated chitosan as well as the production of ROS upon illumination in visible light, causing oxidative stress against microbes found in hospital effluent.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1751-8741, 1751-875XeISSN: 1751-875XDOI: 10.1049/iet-nbt.2019.0149Titel-ID: cdi_doaj_primary_oai_doaj_org_article_894f7d79cc91437a9f0414938f68c45b
- Format
- –
- Schlagworte
- annealing, annealing process, antibacterial activity, antibiotic resistant bacteria, Bacteria - radiation effects, bactericidal activity, CeO2 :Fe, Cerium - chemistry, Cerium - pharmacology, cerium compounds, chemical engineering, Coated Materials, Biocompatible - chemical synthesis, Coated Materials, Biocompatible - chemistry, Coated Materials, Biocompatible - pharmacology, diseases, disease‐causing microbes, Disinfection - methods, effluents, efflux pump inhibition, Foeniculum - chemistry, Foeniculum vulgare seeds extract, Green Chemistry Technology, health and safety, hospital effluent, Hospitals, hydroxyl ions, hydroxyl radical, iron, Iron - chemistry, Iron - pharmacology, Light, Metal Nanoparticles - chemistry, Microbial Sensitivity Tests, Microbial Viability - radiation effects, microorganisms, nanofabrication, nanoparticles, Nanoparticles - chemistry, oxidative stress, particle size, photochemistry, photoinactivation, Phototherapy - methods, reactive oxygen species, Reactive Oxygen Species - chemistry, Reactive Oxygen Species - radiation effects, renewable materials, Research Article, scanning electron microscopy, Seeds - chemistry, Sewage - microbiology, singlet oxygen, size 30.0 nm, solubility, Sulfhydryl Compounds - chemistry, Sulfhydryl Compounds - pharmacology, Sulfur Compounds - chemistry, Sulfur Compounds - pharmacology, temperature 200.0 degC, thiolated chitosan, thiolated iron‐doped nanoceria, visible light, Waste Water - microbiology, wastewater treatment, Water Purification - methods, X‐ray diffraction