Scientific reports, 2018-04, Vol.8 (1), p.6737-14, Article 6737
2018
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Details
- Autor(en) / Beteiligte
- Titel
- Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
- Ist Teil von
- Scientific reports, 2018-04, Vol.8 (1), p.6737-14, Article 6737
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2018
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Whole-transcriptome analysis and western blotting of sauchinone-treated HepG2 cells demonstrated that sauchinone regulated genes relevant to cholesterol metabolism and synthesis. In particular, it was found that the expression of proprotein convertase subtilisin/kexin type 9 (PCSK9) was downregulated, and the expression of low density lipoprotein receptor (LDLR) was upregulated in sauchinone-treated HepG2 cells. Consequently, LDL-cholesterol (LDL-C) uptake was increased. As a transcriptional regulator of PCSK9 expression, sterol regulatory elements binding protein-2 (SREBP-2) was proposed by transcriptome analysis and western blotting. Oral administration of sauchinone increased hepatic LDLR through PCSK9 inhibition in obese mice and showed the reduced serum LDL-C levels and downstream targets of SREBP-2. Thus, it is evident that sauchinone reduces hepatic steatosis by downregulating the expression of hepatic PCSK9 via SREBP-2.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-018-24935-6Titel-ID: cdi_doaj_primary_oai_doaj_org_article_88c1acd2a8594bb8aeaab7ad3b865b1a
- Format
- –
- Schlagworte
- Cholesterol, Fatty liver, Gene expression, Gene regulation, Homeostasis, Kexin, Lipid metabolism, Liver, Low density lipoprotein, Low density lipoprotein receptors, Oral administration, Proprotein convertases, Receptor density, Regulatory sequences, Rodents, Steatosis, Sterol regulatory element-binding protein, Subtilisin, Transcription, Western blotting