Details

Autor(en) / Beteiligte

• Sonnentag, Steffen J.
• Jenne, Felix
• Orian-Rousseau, Véronique
• Nesterov-Mueller, Alexander

Titel

High-throughput screening for cell binding and repulsion peptides on multifunctionalized surfaces

Ist Teil von

• Communications biology, 2024-07, Vol.7 (1), p.870-8, Article 870

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• The adhesion of cells to the extracellular matrix engages cell surface receptors such as integrins, proteoglycans and other types of cell adhesion molecules such as CD44. To closely examine the determinants of cell adhesion, herein we describe the generation of high-density peptide arrays and test the growth of cells on these multifunctionalized surfaces. The peptide library used consists of over 11,000 different sequences, either random or derived from existing proteins. By applying this screen to SW620 mCherry colorectal cancer cells, we select for peptides with both maximum cell adhesion and maximum cell repulsion. All of these extreme properties are based on unique combinations of amino acids. Here, we identify peptides with maximum cell repulsion on secreted frizzled- and Dickkopf-related proteins. Peptides with strong cell repulsion are found at the poles of the TNF-alpha homotrimer. The formation of cellular patterns on alternating highly repulsive and adhesive peptides are examined. Our screen allows the identification of peptides suitable for biomedical and tissue engineering applications. A screen on high-density peptide arrays identified peptides with high cell-adhesion/ repulsion properties. Peptides with high repulsive properties as those found in TNF-alpha are particularly suitable for biomedical/bioengineering applications.