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Pseudomonas aeruginosa Quorum-Sensing and Type VI Secretion System Can Direct Interspecific Coexistence During Evolution
Ist Teil von
Frontiers in microbiology, 2018-10, Vol.9, p.2287-2287
Ort / Verlag
Switzerland: Frontiers Media S.A
Erscheinungsjahr
2018
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
It is reported that a wide range of bacterial infections are polymicrobial, and the members in a local microcommunity can influence the growth of neighbors through physical and chemical interactions.
is an important opportunistic pathogen that normally causes a variety of acute and chronic infections, and clinical evidences suggest that
can be frequently coisolated with other pathogens from the patients with chronic infections. However, the interspecific interaction and the coexisting mechanism of
with coinfecting bacterial species during evolution still remain largely unclear. In this study, the relationships of
with other Gram-positive (
) and Gram-negative (
) are investigated by using a series of on-plate proximity assay,
coevolution assay, and RNA-sequencing. We find that although the development of a quorum-sensing system contributes
a significant growth advantage to compete with
and
, the quorum-sensing regulation of
will be decreased during evolution and thus provides a basis for the formation of interspecific coexistence. The results of comparative transcriptomic analyses suggest that the persistent survival of
in the microcommunity has no significant effect on the intracellular transcriptional pattern of
, while a more detailed competition happens between
and
. Specifically, the population of
with decreased quorum-sensing regulation can still restrict the proportion increase of
by enhancing the type VI secretion system-elicited cell aggressivity during further coevolution. These findings provide a general explanation for the formation of a dynamic stable microcommunity consisting of more than two bacterial species, and may contribute to the development of population biology and clinical therapy.