Genomics, proteomics & bioinformatics, 2014-06, Vol.12 (3), p.111-119
2014
Details
- Autor(en) / Beteiligte
- Titel
- Adolescent Mouse Takes on An Active Transcriptomic Expression During Postnatal Cerebral Development
- Ist Teil von
- Genomics, proteomics & bioinformatics, 2014-06, Vol.12 (3), p.111-119
- Ort / Verlag
- China: Elsevier Ltd
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Postnatal cerebral development is a complicated biological process precisely controlled by multiple genes. To understand the molecular mechanism of cerebral development, we compared dynamics of mouse cerebrum transcriptome through three developmental stages using high-throughput RNA-seq technique. Three libraries were generated from the mouse cerebrum at infancy, adolescence and adulthood, respectively. Consequently, 44,557,729 （infancy）, 59,257,530 （adolescence） and 72,729,636 （adulthood） reads were produced, which were assembled into 15,344, 16,048 and 15,775 genes, respectively. We found that the overall gene expression level increased from infancy to adolescence and decreased later on upon reaching adulthood. The adolescence cerebrum has the most active gene expression, with expression of a large number of reg- ulatory genes up-regulated and some crucial pathways activated. Transcription factor （TF） analysis suggested the similar dynamics as expression profiling, especially those TFs functioning in neurogenesis differentiation, oligodendrocyte lineage determination and circadian rhythm regulation. Moreover, our data revealed a drastic increase in myelin basic protein （MBP）-coding gene expression in adolescence and adulthood, suggesting that the brain myelin may be generated since
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1672-0229eISSN: 2210-3244DOI: 10.1016/j.gpb.2014.04.004Titel-ID: cdi_doaj_primary_oai_doaj_org_article_8471c33067d34485abf2deb3740593b2
- Format
- –
- Schlagworte
- Age Factors, Animals, Cerebrum, Cerebrum - growth & development, Cerebrum - metabolism, Circadian Rhythm - genetics, Gene expression, Gene Expression Profiling - methods, Gene Expression Regulation, Developmental, Genes, Regulator, High-Throughput Nucleotide Sequencing, High-throughput sequencing, Mice, Mice, Inbred BALB C, Molecular Sequence Annotation, Myelin Basic Protein - genetics, Myelin Basic Protein - metabolism, Neurogenesis - genetics, Oligodendroglia - cytology, Oligodendroglia - metabolism, Original Research, Postnatal development, Transcription Factors - genetics, Transcription Factors - metabolism, Transcriptome, 基因表达水平, 多基因控制, 大脑, 小鼠, 生后, 转录表达, 青少年, 髓鞘碱性蛋白