Details

Autor(en) / Beteiligte

• Matuz‐Mares, Deyamira
• Matus‐Ortega, Genaro
• Cárdenas‐Monroy, Christian
• Romero‐Aguilar, Lucero
• Villalobos‐Rocha, Juan Carlos
• Vázquez‐Meza, Héctor
• Guerra‐Sánchez, Guadalupe
• Peña‐Díaz, Antonio
• Pardo, Juan Pablo

Titel

Expression of alternative NADH dehydrogenases (NDH‐2) in the phytopathogenic fungus Ustilago maydis

Ist Teil von

• FEBS open bio, 2018-08, Vol.8 (8), p.1267-1279

Ort / Verlag

England: John Wiley & Sons, Inc

Erscheinungsjahr

2018

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Type 2 alternative NADH dehydrogenases (NDH‐2) participate indirectly in the generation of the electrochemical proton gradient by transferring electrons from NADH and NADPH into the ubiquinone pool. Due to their structural simplicity, alternative NADH dehydrogenases have been proposed as useful tools for gene therapy of cells with defects in the respiratory complex I. In this work, we report the presence of three open reading frames, which correspond to NDH‐2 genes in the genome of Ustilago maydis. These three genes were constitutively transcribed in cells cultured in YPD and minimal medium with glucose, ethanol, or lactate as carbon sources. Proteomic analysis showed that only two of the three NDH‐2 were associated with isolated mitochondria in all culture media. Oxygen consumption by permeabilized cells using NADH or NADPH was different for each condition, opening the possibility of posttranslational regulation. We confirmed the presence of both external and internal NADH dehydrogenases, as well as an external NADPH dehydrogenase insensitive to calcium. Higher oxygen consumption rates were observed during the exponential growth phase, suggesting that the activity of NADH and NADPH dehydrogenases is coupled to the dynamics of cell growth. The Ustilago maydis genome contains three genes coding for mitochondrial alternative NAD(P)H dehydrogenases (NDH‐2). However, only two of these proteins were found in mitochondria. Higher oxygen consumption rates were observed during the exponential growth phase, suggesting that the activity of NADH and NADPH dehydrogenases is coupled to the dynamics of cell growth.