Details

Autor(en) / Beteiligte

• Park, Geun Woo
• Ng, Terry Fei Fan
• Freeland, Amy L
• Marconi, Vincent C
• Boom, Julie A
• Staat, Mary A
• Montmayeur, Anna Maria
• Browne, Hannah
• Narayanan, Jothikumar
• Payne, Daniel C
• Cardemil, Cristina V
• Treffiletti, Aimee
• Vinjé, Jan

Titel

CrAssphage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands

Ist Teil von

• Emerging infectious diseases, 2020-08, Vol.26 (8), p.1731-1739

Ort / Verlag

United States: U.S. National Center for Infectious Diseases

Erscheinungsjahr

2020

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• CrAssphage is a recently discovered human gut-associated bacteriophage. To validate the potential use of crAssphage for detecting human fecal contamination on environmental surfaces and hands, we tested stool samples (n = 60), hand samples (n = 30), and environmental swab samples (n = 201) from 17 norovirus outbreaks for crAssphage by real-time PCR. In addition, we tested stool samples from healthy persons (n = 173), respiratory samples (n = 113), and animal fecal specimens (n = 68) and further sequenced positive samples. Overall, we detected crAssphage in 71.4% of outbreak stool samples, 48%-68.5% of stool samples from healthy persons, 56.2% of environmental swabs, and 60% of hand rinse samples, but not in human respiratory samples or animal fecal samples. CrAssphage sequences could be grouped into 2 major genetic clusters. Our data suggest that crAssphage could be used to detect human fecal contamination on environmental surfaces and hands.