Details

Autor(en) / Beteiligte

• Wenski, Sebastian L
• Cimen, Harun
• Berghaus, Natalie
• Fuchs, Sebastian W
• Hazir, Selcuk
• Bode, Helge B

Titel

Fabclavine diversity in Xenorhabdus bacteria

Ist Teil von

• Beilstein journal of organic chemistry, 2020-05, Vol.16 (1), p.956-965

Ort / Verlag

Germany: Beilstein-Institut zur Föerderung der Chemischen Wissenschaften

Erscheinungsjahr

2020

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The global threat of multiresistant pathogens has to be answered by the development of novel antibiotics. Established antibiotic applications are often based on so-called secondary or specialized metabolites (SMs), identified in large screening approaches. To continue this successful strategy, new sources for bioactive compounds are required, such as the bacterial genera or . In these strains, fabclavines are widely distributed SMs with a broad-spectrum bioactivity. Fabclavines are hybrid SMs derived from nonribosomal peptide synthetases (NRPS), polyunsaturated fatty acid (PUFA), and polyketide synthases (PKS). Selected and mutant strains were generated applying a chemically inducible promoter in front of the suggested fabclavine ( ) biosynthesis gene cluster (BGC), followed by the analysis of the occurring fabclavines. Subsequently, known and unknown derivatives were identified and confirmed by MALDI-MS and MALDI-MS experiments in combination with an optimized sample preparation. This led to a total number of 22 novel fabclavine derivatives in eight strains, increasing the overall number of fabclavines to 32. Together with the identification of fabclavines as major antibiotics in several entomopathogenic strains, our work lays the foundation for the rapid fabclavine identification and dereplication as the basis for future work of this widespread and bioactive SM class.