Details

Autor(en) / Beteiligte

• Chen, Tianyi
• Pu, Meng
• Subramanian, Sundharraman
• Kearns, Dan
• Rowe-Magnus, Dean
• Waters, Christopher M.
• Ruby, Edward G.

Titel

PlzD modifies Vibrio vulnificus foraging behavior and virulence in response to elevated c-di-GMP

Ist Teil von

• mBio, 2023-10, Vol.14 (5), p.e0153623-e0153623

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Many free-swimming bacteria propel themselves through liquid using rotary flagella, and mounting evidence suggests that the inhibition of flagellar rotation initiates biofilm formation, a sessile lifestyle that is a nearly universal surface colonization paradigm in bacteria. In general, motility and biofilm formation are inversely regulated by the intracellular second messenger bis-(3´-5´)-cyclic dimeric guanosine monophosphate (c-di-GMP). Here, we identify a protein, PlzD, bearing a conserved c-di-GMP binding PilZ domain that localizes to the flagellar pole in a c-di-GMP-dependent manner and alters the foraging behavior, biofilm, and virulence characteristics of the opportunistic human pathogen, . Our data suggest that PlzD interacts with components of the flagellar stator to decrease bacterial swimming speed and changes in swimming direction, and these activities are enhanced when cellular c-di-GMP levels are elevated. These results reveal a physical link between a second messenger (c-di-GMP) and an effector (PlzD) that promotes transition from a motile to a sessile state in .