Details

Autor(en) / Beteiligte

• Villagra-Blanco, R
• Silva, L M R
• Aguilella-Segura, A
• Arcenillas-Hernández, I
• Martínez-Carrasco, C
• Seipp, A
• Gärtner, U
• Ruiz de Ybañez, R
• Taubert, A
• Hermosilla, C

Titel

Bottlenose dolphins ( Tursiops truncatus ) do also cast neutrophil extracellular traps against the apicomplexan parasite Neospora caninum

Ist Teil von

• International journal for parasitology. Parasites and wildlife, 2017-12, Vol.6 (3), p.287-294

Ort / Verlag

England: Elsevier

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Neutrophil extracellular traps (NETs) are web-like structures composed of nuclear DNA decorated with histones and cytoplasmic peptides which antiparasitic properties have not previously been investigated in cetaceans. Polymorphonuclear neutrophils (PMN) were isolated from healthy bottlenose dolphins ( ), and stimulated with tachyzoites and the NETs-agonist zymosan. interactions of PMN with the tachyzoites resulted in rapid extrusion of NETs. For the demonstration and quantification of cetacean NETs, extracellular DNA was stained by using either Sytox Orange or Pico Green . Scanning electron microscopy (SEM) and fluorescence analyses demonstrated PMN-derived release of NETs upon exposure to tachyzoites of Co-localization studies of induced cetacean NETs proved the presence of DNA adorned with histones (H1, H2A/H2B, H3, H4), neutrophil elastase (NE), myeloperoxidase (MPO) and pentraxin (PTX) confirming the molecular properties of mammalian NETosis. Dolphin-derived -NETosis were efficiently suppressed by DNase I and diphenyleneiodonium (DPI) treatments. Our results indicate that cetacean-derived NETs represent an ancient, conserved and relevant defense effector mechanism of the host innate immune system against and probably other related neozoan parasites circulating in the marine environment.