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Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
Ist Teil von
Frontiers in microbiology, 2022-10, Vol.13, p.1034779-1034779
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2022
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Bacterial leaf blight caused by Gram-negative pathogen
Xanthomonas oryzae
pv.
oryzae
(
Xoo
) is one of the most destructive bacterial diseases on rice. Due to the resistance, toxicity and environmental issues of chemical bactericides, new biological strategies are still in need. Although peptaibols produced by
Trichoderma
spp. can inhibit the growth of several Gram-positive bacteria and plant fungal pathogens, it still remains unclear whether peptaibols have anti-
Xoo
activity to control bacterial leaf blight on rice. In this study, we evaluated the antibacterial effects of Trichokonins A (TKA), peptaibols produced by
Trichoderma longibrachiatum
SMF2, against
Xoo
. The
in vitro
antibacterial activity analysis showed that the growth of
Xoo
was significantly inhibited by TKA, with a minimum inhibitory concentration of 54 μg/mL and that the three TKs in TKA all had remarkable anti-
Xoo
activity. Further inhibitory mechanism analyses revealed that TKA treatments resulted in the damage of
Xoo
cell morphology and the release of intracellular substances, such as proteins and nucleic acids, from
Xoo
cells, suggesting the damage of the permeability of
Xoo
cell membrane by TKA. Pathogenicity analyses showed that the lesion length on rice leaf was significantly reduced by 82.2% when treated with 27 μg/mL TKA. This study represents the first report of the antibacterial activity of peptaibols against a Gram-negative bacterium. Thus, TKA can be of a promising agent in controlling bacterial leaf blight on rice.