Details

Autor(en) / Beteiligte

• Bangasser, Benjamin L
• Shamsan, Ghaidan A
• Chan, Clarence E
• Opoku, Kwaku N
• Tüzel, Erkan
• Schlichtmann, Benjamin W
• Kasim, Jesse A
• Fuller, Benjamin J
• McCullough, Brannon R
• Rosenfeld, Steven S
• Odde, David J

Titel

Shifting the optimal stiffness for cell migration

Ist Teil von

• Nature communications, 2017-05, Vol.8 (1), p.15313-15313, Article 15313

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2017

Link zum Volltext

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches: embryonic chick forebrain neurons (ECFNs; optimum ∼1 kPa) and U251 glioma cells (optimum ∼100 kPa). In addition, the model predicts, and experiments confirm, that the stiffness optimum of U251 glioma cell migration, morphology and F-actin retrograde flow rate can be shifted to lower stiffness by simultaneous drug inhibition of myosin II motors and integrin-mediated adhesions.