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Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of ZmMs20 Gene Regulating Male Fertility in Maize
Ist Teil von
International journal of molecular sciences, 2019-03, Vol.20 (6), p.1411
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2019
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Genic male sterility (GMS) mutant is a useful germplasm resource for both theory research and production practice. The identification and characterization of GMS genes, and assessment of male-sterility stability of GMS mutant under different genetic backgrounds in
(maize) have (1) deepened our understanding of the molecular mechanisms controlling anther and pollen development, and (2) enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for hybrid breeding. Here, we reported a complete GMS mutant (
), which displays abnormal anther cuticle and pollen development. Its fertility restorer gene
was found to be a new allele of
encoding a glucose methanol choline (GMC) oxidoreductase involved in lipid metabolism in anther. Phylogenetic and microsynteny analyses showed that ZmMs20 was conserved among gramineous species, which provide clues for creating GMS materials in other crops. Additionally, among the 17 maize cloned GMS genes,
was found to be similar to the expression patterns of
,
,
,
, and
genes, which will give some clues for deciphering their functional relationships in regulating male fertility. Finally, two functional markers of
were developed and tested for creating maize
male-sterility lines in 353 genetic backgrounds, and then an artificial maintainer line of
GMS mutation was created by using
gene,
mutant, and BMS system. This work will promote our understanding of functional mechanisms of male fertility and facilitate molecular breeding of
male-sterility lines for hybrid seed production in maize.