Details

Autor(en) / Beteiligte

• Kondratyeva, Elena I
• Zakharova, Irina N
• Ilenkova, Natalya A
• Klimov, Leonid Ya
• Petrova, Nika V
• Zodbinova, Aisa E
• Zhekaite, Elena K
• Chikunov, Vladimir V
• Dolbnya, Svetlana V
• Voronkova, Anna Yu
• Sherman, Victoria D
• Loshkova, Elena V
• Melyanovskaya, Yuliya L
• Budzinskiy, Roman M
• Kuryaninova, Victoria A
• Kutsev, Sergey I

Titel

Vitamin D Status in Russian Children and Adolescents: Contribution of Genetic and Exogenous Factors

Ist Teil von

• Frontiers in pediatrics, 2020-11, Vol.8, p.583206-583206

Ort / Verlag

Switzerland: Frontiers Media S.A

Erscheinungsjahr

2020

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The problem of vitamin D deficiency is particularly relevant for the entire territory of Russia, since most parts of the country are located above the 42nd geographical latitude and the residents are therefore at risk of vitamin D deficiency. Despite the urgency of the problem, a comprehensive study of the molecular and genetic mechanisms and exogenous factors of vitamin D deficiency in children living in various geographical areas of the Russian Federation has not been conducted. Different variants in the loci of the genes responsible for the synthesis, hydroxylation, and transport of vitamin D (such as , and ), as well as gene polymorphisms may also be associated with the risk of vitamin D deficiency. The aim of this study was to analyze the influence of exogenous factors on the blood levels of 25-hydroxyvitamin D (25(OH)D) in children of three regions of the Russian Federation, as well as the relationship of blood 25(OH)D levels with polymorphic variants of cytochrome P450 genes and gene. We conducted blood 25(OH)D level analysis in 333 healthy children and adolescents in three regions located in different geographical zones of the Russian Federation. We studied the polymorphic variants c.1075A>C (I359L, rs1057910, ) and c.430C>T (R144C, rs1799853, ) in the gene, c.1334T>C (M445T, rs4986910, ), and (c.-392C>T, rs2740574) in the gene, 1846G>A, (rs3892097, ) in the gene, I (NM_000376.2: c.1056T>C; rs731236), I (NM_000376.2:c.2T>C; (rs2228570), and I (NM_000376.2: c.1024+283G>A; rs1544410) in the gene. We also analyzed the influence of exogenous factors on the level of 25(OH)D in children of the three study regions, as well as the relationship of the level of 25(OH)D with variants (c.430C>T; R144C), (c,1075A>C; I359L), (1846G>A), (c.1334T>C), and (c.-392C>T) and rs731236, rs2228570 and rs1544410 in the gene. We found that the blood level of 25(OH)D depended on the geographical location and the number of sunny days per year. The average blood level of 25(OH)D in adolescent boys was statistically significantly lower than in girls of this age group. The level of 25(OH)D also significantly depended on the prophylactic dose of cholecalciferol administered to the subjects. In the study, it was shown that a dose of cholecalciferol ≥1,000 IU per day can achieve a normal level of 25(OH)D in healthy children. We found no statistically significant association between single-nucleotide polymorphic variants of cytochrome P450 genes ( , and ) and blood level of 25(OH)D in the subjects. We also did not find a relationship between the I, I, and I polymorphisms of the gene and serum 25(OH)D concentration. Exogenous factors (time of year, place of residence, and prophylactic administration of cholecalciferol), as well as endogenous factors (age and sex), play a determining role in the development of vitamin D deficiency and insufficiency; in contrast to genetic factors-polymorphic variants of the genes of xenobiotic phase 1 enzymes ( , and ) and the gene-which do not play such role. This study shows the need to create a diagnostic algorithm for Vitamin D deficiency based on the age, season of the year, and prophylactic dose of cholecalciferol.