Details

Autor(en) / Beteiligte

• Alotaibi, Bader S
• Tantry, Bilal Ahmad
• Bandy, Altaf
• Ahmad, Reyaz
• Khursheed, Syed Quibtiya
• Ahmad, Arshid
• Hakami, Mohammed Ageeli
• Shah, Naveed Nazir

Titel

Simultaneous detection of influenza A/B, respiratory syncytial virus, and SARS-CoV-2 in nasopharyngeal swabs by one-tube multiplex reverse transcription polymerase chain reaction

Ist Teil von

• Tropical medicine and infectious disease, 2023-06, Vol.8 (6), p.1-16

Ort / Verlag

Basel, Switzerland: MDPI

Erscheinungsjahr

2023

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The treatment and outcome of respiratory virus infections differ. SARS-CoV-2, as well as other respiratory viruses such as influenza virus (A and B) and respiratory syncytial virus (RSV), require simultaneous, cost-effective, and rapid differential detection. We used a gold standard fivetarget single-step RT-PCR to detect influenza viruses, RSV, and SARS-CoV-2, and this method can be extended to detect influenza virus subtypes. As a result, this five-target single-step RT-PCR method is ideal for differentiating respiratory viruses. The 5' nuclease activity of Taq DNA polymerase is used in the real-time reverse transcription PCR assay. The Taq man fast viral 1-step enzyme is a 4 Master mix and five-target primer probe mix that detects influenza A, influenza B, SARS-CoV- 2 ORF1ab, respiratory syncytial viruses A/B and actin. When compared with TaqMan and Invitrogen superscript III Platinum and the Meril Kit for SARS-CoV-2, the assay demonstrated 100% sensitivity, specificity, and amplification efficiency of 90.1% for target genes. In conclusion, our one-tube multiplex RT-PCR assay offers a rapid and reliable method for the simultaneous detection of influenza A/B, RSV, and SARS-CoV-2 from nasopharyngeal swabs. This assay has the potential to enhance diagnostic capabilities and improve public health responses during respiratory outbreaks, enabling timely interventions and informed decision making.