Details

Autor(en) / Beteiligte

• Morozova, Vera
• Kozlova, Yuliya
• Tikunov, Artem
• Babkin, Igor
• Ushakova, Tatyana
• Bardasheva, Alevtina
• Jdeed, Ghadeer
• Zhirakovskaya, Elena
• Mogileva, Alina
• Netesov, Sergei
• Tikunova, Nina

Titel

Identification, Characterization, and Genome Analysis of Two Novel Temperate Pseudomonas protegens Phages PseuP_222 and PseuP_224

Ist Teil von

• Microorganisms (Basel), 2023-05, Vol.11 (6), p.1456

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2023

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Two novel bacteriophages PseuP_222 and Pseu_224 and their host CEMTC 4060 were isolated from the same sample (Inya river, Siberia). Both phages have siphovirus morphology and belong to lambdoid phages. Comparative genome analysis revealed a low nucleotide and amino acid sequence similarity of PseuP_222 and PseuP_224 between themselves, and between them and other lambdoid phages. Bioinformatics analysis indicated that PseuP_222 and PseuP_224 are members of a genetically diverse group of phages of environmental spp.; this group is distant from a large group of phages. In phylogenetic trees, the positioning of the terminase large subunits, major capsid proteins, tail tape measure proteins, and CI-like repressors of PseuP_222 and PseuP_224 were remote and changed relative to those of the lambda phage and lambdoid phages of spp. However, the nucleoid-associated protein NdpA/YejK and P5-like structural protein from both phages showed high similarity and were not found in lambda phage and other lambdoid phages of spp. Substantial divergences of the PseuP_222 and PseuP_224 genomes and proteomes indicated that the evolutionary history of these phages was mostly independent and they probably began to use one host only recently.