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The main attempt of this study is to isolate, determine potential probiotic properties and enzyme production of some lactic acid bacteria (LAB). Among all isolates, two LAB strains isolated from human mother milk and cottage cheese revealed antimicrobial activity against some tested pathogenic strains. Both isolates inhibited all the tested pathogens except
Escherichia coli
. The two isolates were identified by morphological, biochemical properties and then by 16S rRNA gene sequencing technique as
Lactobacillus acidophilus
SAM1 and
Lactiplantibacillus plantarum
SAM2. Potential probiotic characters were investigated. Both strains survived in relatively low pH and high bile concentrations and were able to grow at 0.5% of pancreatin concentrations. Their growth decreased by increasing phenol from 0.2% till 0.5%. Both strains did not show hemolytic activity. Coaggregation potential was exhibited by the two strains against
Staphylococcus aureus
and
Candida albicans
. Hydrophobicity of
Lactobacillus acidophilus
SAM1 and
Lactiplantibacillus plantarum
SAM2, with ethyl acetate; were 88.1% and 82.8%, respectively.
Lactobacillus acidophilus
SAM1 was susceptible to Ampicillin, Penicillin, Erythromycin, Ciprofloxacin and Tetracycline; on the contrary, it resists Vancomycin and Cefoxitin; while
Lactiplantibacillus plantarum
SAM2 resists all tested antibiotics. Maximum growth was achieved using glucose as a carbon source and yeast extract as nitrogen source for both strains; however, glucose is the most preferred carbon source for microorganisms and it prevents the uptake of carbon from other sources like yeast by catabolite repression mechanism.
Lactobacillus acidophilus
SAM1 produces lipase enzyme, while
Lactiplantibacillus plantarum
SAM2 produces amylase and protease.