Details

Autor(en) / Beteiligte

• Albrecht, Martin
• Hummitzsch, Lars
• Rusch, Rene
• Heß, Katharina
• Steinfath, Markus
• Cremer, Jochen
• Lichte, Frank
• Fändrich, Fred
• Berndt, Rouven
• Zitta, Karina

Titel

Characterization of large extracellular vesicles (L-EV) derived from human regulatory macrophages (Mreg): novel mediators in wound healing and angiogenesis?

Ist Teil von

• Journal of translational medicine, 2023-01, Vol.21 (1), p.61-61, Article 61

Ort / Verlag

England: BioMed Central

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Large extracellular vesicles (L-EV) with a diameter between 1 and 10 µm are released by various cell types. L-EV contain and transport active molecules which are crucially involved in cell to cell communication. We have shown that secretory products of human regulatory macrophages (Mreg) bear pro-angiogenic potential in-vitro and our recent findings show that Mreg cultures also contain numerous large vesicular structures similar to L-EV with so far unknown characteristics and function. To characterize the nature of Mreg-derived L-EV (L-EV ) and to gain insights into their role in wound healing and angiogenesis. Mreg were differentiated using blood monocytes from healthy donors (N = 9) and L-EV were isolated from culture supernatants by differential centrifugation. Characterization of L-EV was performed by cell/vesicle analysis, brightfield/transmission electron microscopy (TEM), flow cytometry and proteome profiling arrays. The impact of L-EV on wound healing and angiogenesis was evaluated by means of scratch and in-vitro tube formation assays. Mreg and L-EV show an average diameter of 13.73 ± 1.33 µm (volume: 1.45 ± 0.44 pl) and 7.47 ± 0.75 µm (volume: 0.22 ± 0.06 pl) respectively. Flow cytometry analyses revealed similarities between Mreg and L-EV regarding their surface marker composition. However, compared to Mreg fewer L-EV were positive for CD31 (P < 0.01), CD206 (P < 0.05), CD103 (P < 0.01) and CD45 (P < 0.05). Proteome profiling suggested that L-EV contain abundant amounts of pro-angiogenic proteins (i.e. interleukin-8, platelet factor 4 and serpin E1). From a functional point of view L-EV positively influenced in-vitro wound healing (P < 0.05) and several pro-angiogenic parameters in tube formation assays (all segment associated parameters, P < 0.05; number of meshes, P < 0.05). L-EV with regenerative and pro-angiogenic potential can be reproducibly isolated from in-vitro cultured human regulatory macrophages. We propose that L-EV could represent a putative therapeutic option for the treatment of chronic wounds and ischemia-associated diseases.