Details

Autor(en) / Beteiligte

• Shao, Yong
• Wang, Xiao-Bo
• Zhang, Jin-Jin
• Li, Ming-Li
• Wu, Shou-Song
• Ma, Xi-Yao
• Wang, Xue
• Zhao, Hui-Fang
• Li, Yuan
• Zhu, Helen He
• Irwin, David M
• Wang, De-Peng
• Zhang, Guo-Jie
• Ruan, Jue
• Wu, Dong-Dong

Titel

Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration

Ist Teil von

• Nature communications, 2020-05, Vol.11 (1), p.2656-2656, Article 2656

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The earthworm is particularly fascinating to biologists because of its strong regenerative capacity. However, many aspects of its regeneration in nature remain elusive. Here we report chromosome-level genome, large-scale transcriptome and single-cell RNA-sequencing data during earthworm (Eisenia andrei) regeneration. We observe expansion of LINE2 transposable elements and gene families functionally related to regeneration (for example, EGFR, epidermal growth factor receptor) particularly for genes exhibiting differential expression during earthworm regeneration. Temporal gene expression trajectories identify transcriptional regulatory factors that are potentially crucial for initiating cell proliferation and differentiation during regeneration. Furthermore, early growth response genes related to regeneration are transcriptionally activated in both the earthworm and planarian. Meanwhile, single-cell RNA-sequencing provides insight into the regenerative process at a cellular level and finds that the largest proportion of cells present during regeneration are stem cells.