Details

Autor(en) / Beteiligte

• Aroutcheva, A A
• Simoes, J A
• Faro, S

Titel

Antimicrobial protein produced by vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis

Ist Teil von

• Infectious Diseases in Obstetrics and Gynecology, 2001, Vol.2001 (1), p.33-39

Ort / Verlag

Egypt: Hindawi Limiteds

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective: To isolate bacteriocin from a vaginal strain of Lactobacillus acidophilus. Methods: L. acidophilus 160 was grown on two media. The first was MRS broth for 18 hours; the cells were harvested, washed, and placed into a chemically defined medium. The second medium resembled vaginal fluid minus protein. Bacteriocin was precipitated from both media using ammonium sulfate. The growth-inhibiting activity of bacteriocin was determined by a bioassay using nine different isolates of Gardnerella vaginalis. Results: MRS broth is not a suitable medium for extracting bacteriocin, because it binds with Tween 80. Bacteriocin was isolated, without contaminating constituents, from chemically defined medium and identified as a single band by electrophoresis. Bacteriocin has a molecular weight of 3.8 kDa. All nine isolates of Gardnerella were inhibited by the bacteriocin isolated from L. acidophilus 160. Conclusions: Bacteriocin produced by L. acidophilus 160 was isolated from the chemically defined medium (starvation medium) in a partially pure form. L. acidophilus 160 bacteriocin inhibited growth of all nine isolates of Gardnerella vaginalis.