Details

Autor(en) / Beteiligte

• Liu, Xin
• Risbakk, Sanne
• Almeida Carvalho, Patricia
• Yang, Mingyi
• Hoff Backe, Paul
• Bjørås, Magnar
• Norby, Truls
• Chatzitakis, Athanasios

Titel

Immobilization of FeFe-hydrogenase on black TiO2 nanotubes as biocathodes for the hydrogen evolution reaction

Ist Teil von

• Electrochemistry communications, 2022-02, Vol.135, p.107221, Article 107221

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• •FeFe-hydrogenase enzymes have been successfully immobilized on black TiO2 nanotubes.•Enzymes attach on readily accessible rather than inner and confined surfaces.•Direct electron transfer between FeFe-hydrogenase and black TiO2 is realized.•Optimized nanotube aspect ratio can possibly “shield” O2-intolerant enzymes. Hydrogenases are attractive biocatalysts for utilization in electrochemical devices as potential replacement for Pt in hydrogen evolving electrodes. In this work, we investigate the immobilization of ferredoxin tagged FeFe-hydrogenase (Fd-HydA1) on black TiO2 nanotubes (bTNTs), with uniform nanotube opening diameters of 140 nm. By utilizing an immunogold labelling method, we show that the enzymes attach on the top surface of the bTNTs film rather than on the inner nanotube walls, reflecting the difficulty to insert enzymes into high aspect-ratio nanomaterials for O2-shielding. Nevertheless, cyclic voltammetry demonstrates direct electron transfer between Fd-HydA1 and bTNTs for the hydrogen evolution reaction (HER) in neutral media. This work provides new insight towards design of new nanostructured electrodes for enzyme immobilization.