Details

Autor(en) / Beteiligte

• Petkov, Stefan
• Bekele, Yonas
• Lakshmikanth, Tadepally
• Hejdeman, Bo
• Zazzi, Maurizio
• Brodin, Petter
• Chiodi, Francesca

Titel

High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood

Ist Teil von

• Scientific reports, 2020-11, Vol.10 (1), p.20425-20425, Article 20425

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45 T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45 cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45 T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45 T cells may contribute to their exhaustion.