Molecules (Basel, Switzerland), 2020-04, Vol.25 (8), p.2003
2020
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- Autor(en) / Beteiligte
- Titel
- Combretum quadrangulare Extract Attenuates Atopic Dermatitis-Like Skin Lesions through Modulation of MAPK Signaling in BALB/c Mice
- Ist Teil von
- Molecules (Basel, Switzerland), 2020-04, Vol.25 (8), p.2003
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Atopic dermatitis (AD) is a chronic inflammatory disease. ( ) is used as a traditional medicine to improve various pathologies in Southeast Asia. In this study, we investigated the effects of ethanol extract (CQ) on 1-chloro-2,4-dinitrobenzene (DNCB)-induced AD like skin lesions in BALB/c mice. After administration with CQ (100, 200, and 400 mg/kg) for 6 weeks, AD symptoms, protein expression, immunoglobulin E (IgE), thymus and activation-regulated chemokine (TARC), and ceramidase level were measured in skin lesions of DNCB-induced BALB/c mice. CQ group improved the dermatitis score, skin pH, transepidermal water loss (TEWL), and skin hydration. Furthermore, histological analysis revealed that CQ attenuated the increased epidermal thickness and infiltration of mast cells caused by DNCB. CQ also increased the expression of filaggrin, and reduced the expression of ceramidase, serum IgE level, and the number of eosinophils. CQ effectively inhibited cytokines and chemokines such as interleukin (IL)-6, IL-13, TARC, and thymic stromal lymphopoietin (TSLP) at the mRNA levels, as well as the activation of mitogen-activated protein kinase (MAPK), including extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 in the skin lesions. Taken together, these findings demonstrate that CQ may be an effective treatment of AD-like skin lesions by inhibiting the expression of inflammatory mediators via the MAPK signaling pathways.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1420-3049eISSN: 1420-3049DOI: 10.3390/molecules25082003Titel-ID: cdi_doaj_primary_oai_doaj_org_article_76a0444f94ab43a995e1109e424bf61f
- Format
- –
- Schlagworte
- 1-Chloro-2,4-dinitrobenzene, Activation, Animals, Atopic dermatitis, c-Jun protein, CCL17 protein, Ceramidase, Chemokines, Chromatography, High Pressure Liquid, Combretum, Combretum - chemistry, Combretum quadrangulare, Cytokines, Cytokines - metabolism, Dermatitis, Dermatitis, Atopic - drug therapy, Dermatitis, Atopic - etiology, Dermatitis, Atopic - metabolism, Dermatitis, Atopic - pathology, Disease, Disease Models, Animal, Drug dosages, Eczema, Eosinophils, Ethanol, Extracellular signal-regulated kinase, Filaggrin, Flavonoids, Hydration, Immunoglobulin E, Immunohistochemistry, inflammation, Inflammation Mediators - metabolism, Inflammatory diseases, Interleukin 13, JNK protein, Kinases, Lesions, Leukocytes (eosinophilic), MAP kinase, MAP Kinase Signaling System - drug effects, Mast cells, Mice, Mice, Inbred BALB C, mitogen-activated protein kinase, mRNA, Phosphorylation, Plant Extracts - chemistry, Plant Extracts - pharmacology, Protein kinase, Proteins, Signs and symptoms, Skin, Skin - drug effects, Skin - metabolism, Skin - pathology, Skin diseases, Skin lesions, Thymic stromal lymphopoietin, Thymus, Transcription factors, Water loss