eLife, 2019-11, Vol.8
2019
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex
- Ist Teil von
- eLife, 2019-11, Vol.8
- Ort / Verlag
- England: eLife Science Publications, Ltd
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cholesterol is a major structural component of the plasma membrane (PM). The majority of PM cholesterol forms complexes with other PM lipids, making it inaccessible for intracellular transport. Transition of PM cholesterol between accessible and inaccessible pools maintains cellular homeostasis, but how cells monitor the accessibility of PM cholesterol remains unclear. We show that endoplasmic reticulum (ER)-anchored lipid transfer proteins, the GRAMD1s, sense and transport accessible PM cholesterol to the ER. GRAMD1s bind to one another and populate ER-PM contacts by sensing a transient expansion of the accessible pool of PM cholesterol via their GRAM domains. They then facilitate the transport of this cholesterol via their StART-like domains. Cells that lack all three GRAMD1s exhibit striking expansion of the accessible pool of PM cholesterol as a result of less efficient PM to ER transport of accessible cholesterol. Thus, GRAMD1s facilitate the movement of accessible PM cholesterol to the ER in order to counteract an acute increase of PM cholesterol, thereby activating non-vesicular cholesterol transport.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.51401Titel-ID: cdi_doaj_primary_oai_doaj_org_article_766733db0b60402c8c57af5b9a78fab3
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Biochemistry and Chemical Biology, Biological Transport - drug effects, Carrier Proteins - chemistry, Carrier Proteins - metabolism, Cell Biology, Cell Membrane - drug effects, Cell Membrane - metabolism, Cell membranes, Cells (Biology), Chlorocebus aethiops, Cholesterol, Cholesterol - metabolism, COS Cells, Endoplasmic reticulum, Endoplasmic Reticulum - drug effects, Endoplasmic Reticulum - metabolism, GRAM domain, HeLa Cells, Homeostasis, Humans, Lipid transfer proteins, Lipids, membrane contact sites, Multiprotein Complexes - metabolism, Mutant Proteins - metabolism, non-vesicular lipid transport, Patient monitoring equipment, Pharmaceutical industry, plasma membrane, Protein Binding - drug effects, Protein Domains, Proteins, Scientific equipment industry, Sirolimus - pharmacology, Sphingomyelins - metabolism, StART-like domain