Details

Autor(en) / Beteiligte

• Sun, Aijun
• Wang, Rui
• Yang, Shuaikang
• Zhu, Xiaojing
• Liu, Ying
• Teng, Man
• Zheng, Luping
• Luo, Jun
• Zhang, Gaiping
• Zhuang, Guoqing

Titel

Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus

Ist Teil von

• Scientific reports, 2021-05, Vol.11 (1), p.11084-11084, Article 11084

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2021

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Marek’s disease virus (MDV) induces severe immunosuppression and lymphomagenesis in the chicken, its natural host, and results in a condition that investigated the pathogenesis of MDV and have begun to focus on the expression profiling of circular RNAs (circRNAs). However, little is known about how the expression of circRNAs is referred to as Marek’s disease. Previous reports have is regulated during MDV replication. Here, we carried out a comprehensive profiling analysis of N6-methyladenosine (m 6 A) modification on the circRNA transcriptome in infected and uninfected chicken embryonic fibroblast (CEF) cells. Methylated RNA immunoprecipitation sequencing (MeRIP-Seq) revealed that m 6 A modification was highly conserved in circRNAs. Comparing to the uninfected group, the number of peaks and conserved motifs were not significantly different in cells that were infected with MDV, although reduced abundance of circRNA m 6 A modifications. However, gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses revealed that the insulin signaling pathway was associated with the regulation of m 6 A modified circRNAs in MDV infection. This is the first report to describe alterations in the transcriptome-wide profiling of m 6 A modified circRNAs in MDV-infected CEF cells.