Details

Autor(en) / Beteiligte

• Wick, Ryan R
• Judd, Louise M
• Cerdeira, Louise T
• Hawkey, Jane
• Méric, Guillaume
• Vezina, Ben
• Wyres, Kelly L
• Holt, Kathryn E

Titel

Trycycler: consensus long-read assemblies for bacterial genomes

Ist Teil von

• Genome Biology, 2021-09, Vol.22 (1), p.266-266, Article 266

Ort / Verlag

England: BioMed Central

Erscheinungsjahr

2021

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• While long-read sequencing allows for the complete assembly of bacterial genomes, long-read assemblies contain a variety of errors. Here, we present Trycycler, a tool which produces a consensus assembly from multiple input assemblies of the same genome. Benchmarking showed that Trycycler assemblies contained fewer errors than assemblies constructed with a single tool. Post-assembly polishing further reduced errors and Trycycler+polishing assemblies were the most accurate genomes in our study. As Trycycler requires manual intervention, its output is not deterministic. However, we demonstrated that multiple users converge on similar assemblies that are consistently more accurate than those produced by automated assembly tools.