Details

Autor(en) / Beteiligte

• Rosario-Rodríguez, Lester J
• Cantres-Rosario, Yadira M
• Carrasquillo-Carrión, Kelvin
• Rosa-Díaz, Alexandra
• Rodríguez-De Jesús, Ana E
• Rivera-Nieves, Verónica
• Tosado-Rodríguez, Eduardo L
• Méndez, Loyda B
• Roche-Lima, Abiel
• Bertrán, Jorge
• Meléndez, Loyda M

Titel

Plasma Proteins Associated with COVID-19 Severity in Puerto Rico

Ist Teil von

• International journal of molecular sciences, 2024-05, Vol.25 (10), p.5426

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Viral strains, age, and host factors are associated with variable immune responses against SARS-CoV-2 and disease severity. Puerto Ricans have a genetic mixture of races: European, African, and Native American. We hypothesized that unique host proteins/pathways are associated with COVID-19 disease severity in Puerto Rico. Following IRB approval, a total of 95 unvaccinated men and women aged 21-71 years old were recruited in Puerto Rico from 2020-2021. Plasma samples were collected from COVID-19-positive subjects ( = 39) and COVID-19-negative individuals ( = 56) during acute disease. COVID-19-positive individuals were stratified based on symptomatology as follows: mild ( = 18), moderate ( = 13), and severe ( = 8). Quantitative proteomics was performed in plasma samples using tandem mass tag (TMT) labeling. Labeled peptides were subjected to LC/MS/MS and analyzed by Proteome Discoverer (version 2.5), Limma software (version 3.41.15), and Ingenuity Pathways Analysis (IPA, version 22.0.2). Cytokines were quantified using a human cytokine array. Proteomics analyses of severely affected COVID-19-positive individuals revealed 58 differentially expressed proteins. Cadherin-13, which participates in synaptogenesis, was downregulated in severe patients and validated by ELISA. Cytokine immunoassay showed that TNF-α levels decreased with disease severity. This study uncovers potential host predictors of COVID-19 severity and new avenues for treatment in Puerto Ricans.