Details

Autor(en) / Beteiligte

• Gómez-Matas, Javier
• Duran-Sanchon, Saray
• Lozano, Juan-José
• Ferrero, Giulio
• Tarallo, Sonia
• Pardini, Barbara
• Naccarati, Alessio
• Castells, Antoni
• Gironella, Meritxell

Titel

SnoRNA profiling in colorectal cancer and assessment of non-invasive biomarker capacity by ddPCR in fecal samples

Ist Teil von

• iScience, 2024-03, Vol.27 (3), p.109283-109283, Article 109283

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Small nucleolar RNAs (snoRNAs) have been identified dysregulated in several pathologies, and these alterations can be detected in tissues and in circulation. The main aim of this study was to analyze the whole snoRNome in advanced colorectal neoplasms and to identify new potential non-invasive snoRNA-based biomarkers in fecal samples by different analytical approaches. SNORA51, SNORD15B, SNORA54, SNORD12B, SNORD12C, SNORD72, SNORD89, and several members of SNORD115 and SNORD116 clusters were consistently deregulated in both tissue sets. After technical validation, SNORA51 and SNORD15B were detected in FIT+ samples. SNORA51 was significantly upregulated in FIT+ samples from CRC patients compared to healthy controls. This upregulation, together with the fecal hemoglobin concentration, was sufficient to identify, among FIT+ individuals, patients with CRC (AUC = 0.86) and individuals with advanced adenomas (AUC = 0.68). These findings portray snoRNAs as an alternative source of candidates for further studies and SNORA51 appears as a potential non-invasive biomarker for CRC detection. [Display omitted] •snoRNA profiles in CRC or AA are different from those of normal mucosa•snoRNA profiles are stable between populations from different countries•snoRNAs can also be detected in fecal samples by ddPCR•SNORA51 can detect CRC cases among FIT+ participants in a CRC screening program Classification Description: Health sciences; Internal medicine; Medical specialty; Medicine; Oncology