Details

Autor(en) / Beteiligte

• Borg, Rebecca
• Herrera, Paul
• Purkiss, Angie
• Cacciottolo, Rebecca
• Cauchi, Ruben J

Titel

Reduced levels of ALS gene DCTN1 induce motor defects in Drosophila

Ist Teil von

• Frontiers in neuroscience, 2023-06, Vol.17, p.1164251-1164251

Ort / Verlag

Switzerland: Frontiers Research Foundation

Erscheinungsjahr

2023

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Amyotrophic lateral sclerosis (ALS) is a rapidly progressive neuromuscular disease that has a strong genetic component. Deleterious variants in the gene are known to be a cause of ALS in diverse populations. encodes the p150 subunit of the molecular motor dynactin which is a key player in the bidirectional transport of cargos within cells. Whether mutations lead to the disease through either a gain or loss of function mechanism remains unresolved. Moreover, the contribution of non-neuronal cell types, especially muscle tissue, to ALS phenotypes in carriers is unknown. Here we show that gene silencing of , the main orthologue of , either in neurons or muscles is sufficient to cause climbing and flight defects in adult flies. We also identify Dred, a protein with high homology to Dctn1 and human DCTN1, that on loss of function also leads to motoric impairments. A global reduction of Dctn1 induced a significant reduction in the mobility of larvae and neuromuscular junction (NMJ) deficits prior to death at the pupal stage. RNA-seq and transcriptome profiling revealed splicing alterations in genes required for synapse organisation and function, which may explain the observed motor dysfunction and synaptic defects downstream of ablation. Our findings support the possibility that loss of function can lead to ALS and underscore an important requirement for DCTN1 in muscle in addition to neurons.