Details

Autor(en) / Beteiligte

• Pintér, György
• Hohmann, Katharina F.
• Grün, J. Tassilo
• Wirmer-Bartoschek, Julia
• Glaubitz, Clemens
• Fürtig, Boris
• Schwalbe, Harald

Titel

Real-time nuclear magnetic resonance spectroscopy in the study of biomolecular kinetics and dynamics

Ist Teil von

• Magnetic Resonance : (Göttingen), 2021, Vol.2 (1), p.291-320

Ort / Verlag

Göttingen: Copernicus GmbH

Erscheinungsjahr

2021

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• The review describes the application of nuclear magnetic resonance (NMR) spectroscopy to study kinetics of folding, refolding and aggregation of proteins, RNA and DNA. Time-resolved NMR experiments can be conducted in a reversible or an irreversible manner. In particular, irreversible folding experiments pose large requirements for (i) signal-to-noise due to the time limitations and (ii) synchronising of the refolding steps. Thus, this contribution discusses the application of methods for signal-to-noise increases, including dynamic nuclear polarisation, hyperpolarisation and photo-CIDNP for the study of time-resolved NMR studies. Further, methods are reviewed ranging from pressure and temperature jump, light induction to rapid mixing to induce rapidly non-equilibrium conditions required to initiate folding.