Nature communications, 2018-03, Vol.9 (1), p.1226-16, Article 1226
2018
Details
- Autor(en) / Beteiligte
- Titel
- A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons
- Ist Teil von
- Nature communications, 2018-03, Vol.9 (1), p.1226-16, Article 1226
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The brain is composed of hundreds of different neuronal subtypes, which largely retain their identity throughout the lifespan of the organism. The mechanisms governing this stability are not fully understood, partly due to the diversity and limited size of clinically relevant neuronal populations, which constitute a technical challenge for analysis. Here, using a strategy that allows for ChIP-seq combined with RNA-seq in small neuronal populations in vivo, we present a comparative analysis of permissive and repressive histone modifications in adult midbrain dopaminergic neurons, raphe nuclei serotonergic neurons, and embryonic neural progenitors. Furthermore, we utilize the map generated by our analysis to show that the transcriptional response of midbrain dopaminergic neurons following 6-OHDA or methamphetamine injection is characterized by increased expression of genes with promoters dually marked by H3K4me3/H3K27me3. Our study provides an in vivo genome-wide analysis of permissive/repressive histone modifications coupled to gene expression in these rare neuronal subtypes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-018-03538-9Titel-ID: cdi_doaj_primary_oai_doaj_org_article_6df7c45a61984546bf5cda6b2f25f8ed
- Format
- –
- Schlagworte
- Animals, Basic Medicine, Brain, Chromatin, Chromatin - metabolism, Chromatin Immunoprecipitation, Comparative analysis, Dopamine receptors, Dopaminergic Neurons - metabolism, Embryos, Female, Gene Expression, Gene Expression Regulation, Gene regulation, Gene Silencing, Genome, Genome-Wide Association Study, Genomes, Histone Code, In vivo methods and tests, Life span, Male, Medical and Health Sciences, Medicin och hälsovetenskap, Medicinska och farmaceutiska grundvetenskaper, Mesencephalon, Methamphetamine, Mice, Mice, Transgenic, Neural stem cells, Neural Stem Cells - metabolism, Neurons, Neurons - metabolism, Neurosciences, Neurovetenskaper, Populations, Raphe nuclei, Ribonucleic acid, RNA, Serotonergic Neurons - metabolism, Serotonin, Stress, Physiological, Transcription