Details

Autor(en) / Beteiligte

• Nogly, Przemyslaw
• James, Daniel
• Wang, Dingjie
• White, Thomas A
• Zatsepin, Nadia
• Shilova, Anastasya
• Nelson, Garrett
• Liu, Haiguang
• Johansson, Linda
• Heymann, Michael
• Jaeger, Kathrin
• Metz, Markus
• Wickstrand, Cecilia
• Wu, Wenting
• Båth, Petra
• Berntsen, Peter
• Oberthuer, Dominik
• Panneels, Valerie
• Cherezov, Vadim
• Chapman, Henry
• Schertler, Gebhard
• Neutze, Richard
• Spence, John
• Moraes, Isabel
• Burghammer, Manfred
• Standfuss, Joerg
• Weierstall, Uwe

Titel

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

Ist Teil von

• IUCrJ, 2015-03, Vol.2 (Pt 2), p.168-176

Ort / Verlag

England: International Union of Crystallography

Erscheinungsjahr

2015

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.