Details

Autor(en) / Beteiligte

• Grebenyuk, Sergei
• Abdel Fattah, Abdel Rahman
• Kumar, Manoj
• Toprakhisar, Burak
• Rustandi, Gregorius
• Vananroye, Anja
• Salmon, Idris
• Verfaillie, Catherine
• Grillo, Mark
• Ranga, Adrian

Titel

Large-scale perfused tissues via synthetic 3D soft microfluidics

Ist Teil von

• Nature communications, 2023-01, Vol.14 (1), p.193-193, Article 193

Ort / Verlag

England: Nature Publishing Group UK

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• The vascularization of engineered tissues and organoids has remained a major unresolved challenge in regenerative medicine. While multiple approaches have been developed to vascularize in vitro tissues, it has thus far not been possible to generate sufficiently dense networks of small-scale vessels to perfuse large de novo tissues. Here, we achieve the perfusion of multi-mm tissue constructs by generating networks of synthetic capillary-scale 3D vessels. Our 3D soft microfluidic strategy is uniquely enabled by a 3D-printable 2-photon-polymerizable hydrogel formulation, which allows for precise microvessel printing at scales below the diffusion limit of living tissues. We demonstrate that these large-scale engineered tissues are viable, proliferative and exhibit complex morphogenesis during long-term in-vitro culture, while avoiding hypoxia and necrosis. We show by scRNAseq and immunohistochemistry that neural differentiation is significantly accelerated in perfused neural constructs. Additionally, we illustrate the versatility of this platform by demonstrating long-term perfusion of developing neural and liver tissue. This fully synthetic vascularization platform opens the door to the generation of human tissue models at unprecedented scale and complexity.