BMC microbiology, 2018-08, Vol.18 (1), p.91-91, Article 91
2018
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- Autor(en) / Beteiligte
- Titel
- Molecular characteristics of Staphylococcus aureus isolates from food surveillance in southwest China
- Ist Teil von
- BMC microbiology, 2018-08, Vol.18 (1), p.91-91, Article 91
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2018
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Staphylococcal food poisoning (SFP) is one of the most common food-borne diseases in the world. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and spa typing methods were used to characterize Staphylococcus aureus isolates from food surveillance during 2013-2015 in southwest China, and Staphylococcal cassette chromosome mec (SCCmec) typing was used for methicillin-resistant S. aureus (MRSA). Isolates were also examined for their antibiotic resistance and carriage of virulence genes. Isolation rate of S. aureus was 2.60% during the three years' surveillance and 29.50% of them were MRSA. All the S. aureus had hla genes (100%), 14.34% of the strains had tst, and 16.73% had PVL. 163 PFGE-SmaI patterns, 41 ST types and 36 spa types were obtained for all the S. aureus. Among them, ST6-t701 (13.15%), ST7-t091 (12.75%), ST59-t437 (9.96%) and ST5-t002 (7.57%) were the prevalent genotypes. Most of MRSA in this study belonged to SCCmec IV and V, accounted for 74.32% and 20.27% respectively. ST6-SCCmec IV-t701 (36.50%) was the most prevalent clone among isolates from food, followed by ST59-SCCmec V-t437 (20.30%), ST5-SCCmec IV-t002 (12.20%) and ST59-SCCmec IV-t437 (12.20%). Some strains had the identical PFGE patterns, ST and spa types with isolates from patients. S. aureus isolated from food in southwest China displayed heterogeneity. Isolates had the same genotype profiles with isolates from patients, indicating high homology.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1471-2180eISSN: 1471-2180DOI: 10.1186/s12866-018-1239-zTitel-ID: cdi_doaj_primary_oai_doaj_org_article_6b94975dc1f544acba580010b0907e8c
- Format
- –
- Schlagworte
- Antibiotic resistance, Antibiotics, Antimicrobial agents, Bacterial Proteins - genetics, Bacterial Toxins - genetics, Bacterial Typing Techniques - methods, Chromosomes, Cloning, Dairy products, DNA, Bacterial, Drug resistance, Drug Resistance, Multiple, Bacterial - genetics, Electrophoresis, Electrophoresis, Gel, Pulsed-Field - methods, Enterotoxins - genetics, Exotoxins - genetics, Food, Food contamination, Food contamination & poisoning, Food Microbiology, Food poisoning, Food products, Food surveillance, Foodborne diseases, Foodborne Diseases - microbiology, Gel electrophoresis, Genes, Genes, Bacterial - genetics, Genotype, Genotypes, Gram-positive bacteria, Health surveillance, Hemolysin Proteins - genetics, Heterogeneity, Histocompatibility antigen HLA, Homology, Humans, Leukocidins - genetics, Methicillin, Methicillin-Resistant Staphylococcus aureus - genetics, Methicillin-Resistant Staphylococcus aureus - isolation & purification, Molecular Epidemiology, Multilocus sequence typing, Multilocus Sequence Typing - methods, Pathogens, Patients, Penicillin-Binding Proteins - genetics, Prevalence, Public health, Pulsed-field gel electrophoresis, Southwest China, Staphylococcal Infections - microbiology, Staphylococcus aureus, Staphylococcus aureus - classification, Staphylococcus aureus - genetics, Staphylococcus aureus - isolation & purification, Staphylococcus infections, Strains (organisms), Superantigens - genetics, Surveillance, Tissue typing, Virulence, Virulence - genetics