eLife, 2013-10, Vol.2, p.e01149-e01149
2013
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- Autor(en) / Beteiligte
- Titel
- Nanoscale protein architecture of the kidney glomerular basement membrane
- Ist Teil von
- eLife, 2013-10, Vol.2, p.e01149-e01149
- Ort / Verlag
- England: eLife Sciences Publications Ltd
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In multicellular organisms, proteins of the extracellular matrix (ECM) play structural and functional roles in essentially all organs, so understanding ECM protein organization in health and disease remains an important goal. Here, we used sub-diffraction resolution stochastic optical reconstruction microscopy (STORM) to resolve the in situ molecular organization of proteins within the kidney glomerular basement membrane (GBM), an essential mediator of glomerular ultrafiltration. Using multichannel STORM and STORM-electron microscopy correlation, we constructed a molecular reference frame that revealed a laminar organization of ECM proteins within the GBM. Separate analyses of domains near the N- and C-termini of agrin, laminin, and collagen IV in mouse and human GBM revealed a highly oriented macromolecular organization. Our analysis also revealed disruptions in this GBM architecture in a mouse model of Alport syndrome. These results provide the first nanoscopic glimpse into the organization of a complex ECM. DOI:http://dx.doi.org/10.7554/eLife.01149.001.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.01149Titel-ID: cdi_doaj_primary_oai_doaj_org_article_6ae1fcc1256c4d77b1238c1bbafde063
- Format
- –
- Schlagworte
- Agrin, Agrin - metabolism, Agrin - ultrastructure, Alport syndrome, Animals, basement membrane, Basement membranes, Biophysics and Structural Biology, Cell Biology, Collagen, Collagen (type IV), Collagen Type IV - metabolism, Collagen Type IV - ultrastructure, Disease Models, Animal, Electron microscopy, Extracellular matrix, Extracellular Matrix - metabolism, Extracellular Matrix - ultrastructure, Glomerular Basement Membrane - metabolism, Glomerular Basement Membrane - ultrastructure, Glomerular Filtration Rate, Humans, Immunoglobulins, Integrins - metabolism, Integrins - ultrastructure, kidney, Kidneys, Labeling, Laminin, Laminin - metabolism, Laminin - ultrastructure, Localization, Macromolecules, Medicine, Mice, Mice, Transgenic, Microscopy, Microscopy, Electron, Transmission - instrumentation, Nephritis, Hereditary - metabolism, Nephritis, Hereditary - pathology, Nephritis, Hereditary - physiopathology, Protein Isoforms - metabolism, Protein Isoforms - ultrastructure, Proteins, Rodents, Stochasticity, Structure-function relationships, super-resolution microscopy, Ultrafiltration, Urine