Details

Autor(en) / Beteiligte

• Radjasa, Ocky Karna
• Steven, Ray
• Humaira, Zalfa
• Dwivany, Fenny Martha
• Nugrahapraja, Husna
• Trinugroho, Joko Pebrianto
• Kristianti, Tati
• Chahyadi, Agus
• Natanael, Yosua
• Priharto, Neil
• Kamarisima
• Sembiring, Farisa Amalia P. B.
• Dwijayanti, Ari
• Kusmita, Lia
• Moeis, Maelita R.
• Suhardi, V. Sri Harjati

Titel

Biosynthetic gene cluster profiling from North Java Sea Virgibacillus salarius reveals hidden potential metabolites

Ist Teil von

• Scientific reports, 2023-11, Vol.13 (1), p.19273-19273, Article 19273

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Abstract Virgibacillus salarius 19.PP.SC1.6 is a coral symbiont isolated from Indonesia's North Java Sea; it has the ability to produce secondary metabolites that provide survival advantages and biological functions, such as ectoine, which is synthesized by an ectoine gene cluster. Apart from being an osmoprotectant for bacteria, ectoine is also known as a chemical chaperone with numerous biological activities such as maintaining protein stability, which makes ectoine in high demand in the market industry and makes it beneficial to investigate V. salarius ectoine. However, there has been no research on genome-based secondary metabolite and ectoine gene cluster characterization from Indonesian marine V. salarius . In this study, we performed a genomic analysis and ectoine identification of V. salarius . A high-quality draft genome with total size of 4.45 Mb and 4426 coding sequence (CDS) was characterized and then mapped into the Cluster of Orthologous Groups (COG) category. The genus Virgibacillus has an "open" pangenome type with total of 18 genomic islands inside the V. salarius 19.PP.SC1.6 genome. There were seven clusters of secondary metabolite-producing genes found, with a total of 80 genes classified as NRPS, PKS (type III), terpenes, and ectoine biosynthetic related genes. The ectoine gene cluster forms one operon consists of ectABC gene with 2190 bp gene cluster length, and is successfully characterized. The presence of ectoine in V. salarius was confirmed using UPLC-MS/MS operated in Multiple Reaction Monitoring (MRM) mode, which indicates that V. salarius has an intact ectoine gene clusters and is capable of producing ectoine as compatible solutes.