Details

Autor(en) / Beteiligte

• Gril, Brunilde
• Paranjape, Anurag N.
• Woditschka, Stephan
• Hua, Emily
• Dolan, Emma L.
• Hanson, Jeffrey
• Wu, Xiaolin
• Kloc, Wojciech
• Izycka-Swieszewska, Ewa
• Duchnowska, Renata
• Pęksa, Rafał
• Biernat, Wojciech
• Jassem, Jacek
• Nayyar, Naema
• Brastianos, Priscilla K.
• Hall, O. Morgan
• Peer, Cody J.
• Figg, William D.
• Pauly, Gary T.
• Robinson, Christina
• Difilippantonio, Simone
• Bialecki, Emilie
• Metellus, Philippe
• Schneider, Joel P.
• Steeg, Patricia S.

Titel

Reactive astrocytic S1P3 signaling modulates the blood–tumor barrier in brain metastases

Ist Teil von

• Nature communications, 2018-07, Vol.9 (1), p.2705-18, Article 2705

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Brain metastases are devastating complications of cancer. The blood–brain barrier (BBB), which protects the normal brain, morphs into an inadequately characterized blood–tumor barrier (BTB) when brain metastases form, and is surrounded by a neuroinflammatory response. These structures contribute to poor therapeutic efficacy by limiting drug uptake. Here, we report that experimental breast cancer brain metastases of low- and high permeability to a dextran dye exhibit distinct microenvironmental gene expression patterns. Astrocytic sphingosine-1 phosphate receptor 3 (S1P3) is upregulated in the neuroinflammatory response of the highly permeable lesions, and is expressed in patients’ brain metastases. S1P3 inhibition functionally tightens the BTB in vitro and in vivo. S1P3 mediates its effects on BTB permeability through astrocytic secretion of IL-6 and CCL2, which relaxes endothelial cell adhesion. Tumor cell overexpression of S1P3 mimics this pathway, enhancing IL-6 and CCL-2 production and elevating BTB permeability. In conclusion, neuroinflammatory astrocytic S1P3 modulates BTB permeability. When brain metastases form, the blood–brain barrier morphs into the blood–tumor barrier (BTB), surrounded by neuroinflammatory response. Here, the authors show that S1P3 is upregulated in neuroinflammatory response in highly BTB permeable lesions, and modulation of S1P3 could impact BTB permeability.