Sensors (Basel, Switzerland), 2011-08, Vol.11 (8), p.7879-7891
2011
Details
- Autor(en) / Beteiligte
- Titel
- Optimizing two-color semiconductor nanocrystal immunoassays in single well microtiter plate formats
- Ist Teil von
- Sensors (Basel, Switzerland), 2011-08, Vol.11 (8), p.7879-7891
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took <3 h. Dose response curves for each target were evaluated in a single immunoassay format and compared to Cy5, a fluorophore commonly used in fluorescent immunoassays, and found to be equivalent. Immunoassays were then performed in a duplex format, demonstrating multiplex detection in a single well with limits of detection equivalent to the single assay format: 9.8 ng/mL chicken IgG and 7.8 ng/mL SEB.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1424-8220eISSN: 1424-8220DOI: 10.3390/s110807879Titel-ID: cdi_doaj_primary_oai_doaj_org_article_68446fc695d34311b5974dddc689c2e5
- Format
- –
- Schlagworte
- Animals, Antibodies, Antibodies - chemistry, bioconjugation, Biosensing Techniques, Carbocyanines - pharmacology, Chemistry, Chickens, Electronic mail systems, Enterotoxins - chemistry, Fluorescent Antibody Technique - methods, Immunoassay, Immunoassay - methods, Immunoglobulin G - chemistry, Laboratories, Luminescence, Medical research, multiplex, nanocrystal (NC), Nanocrystals, Nanoparticles - chemistry, Nanotechnology - methods, Photochemistry - methods, Proteins, quantum dot (QD), Quantum Dots, Reproducibility of Results, semiconductor, Semiconductors, sensor, Sensors, sulfhydryl chemistry