Genome Biology, 2022-03, Vol.23 (1), p.88-88, Article 88
2022
Details
- Autor(en) / Beteiligte
- Titel
- Prime-seq, efficient and powerful bulk RNA sequencing
- Ist Teil von
- Genome Biology, 2022-03, Vol.23 (1), p.88-88, Article 88
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2022
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1474-760X, 1474-7596eISSN: 1474-760XDOI: 10.1186/s13059-022-02660-8Titel-ID: cdi_doaj_primary_oai_doaj_org_article_68399bd59d28488cb10eea8edd8b0b09
- Format
- –
- Schlagworte
- Base Sequence, Bioinformatik och systembiologi (metodutveckling under 10203), Biologi (Medicinska tillämpningar under 3 och lantbruksvetenskapliga under 4), Biology, Costs, Efficiency, Gene expression, Gene Library, Genomes, Genomics, Medicin och hälsovetenskap, Medicinsk genetik, Medicinska och farmaceutiska grundvetenskaper, Method, Naturvetenskap, Power, Power analysis, Ribonucleic acid, RNA, RNA - genetics, RNA-seq, Sequence Analysis, RNA - methods, Transcriptomics, Whole Exome Sequencing