Details

Autor(en) / Beteiligte

• Staunstrup, Nicklas Heine
• Stenderup, Karin
• Mortensen, Sidsel
• Primo, Maria Nascimento
• Rosada, Cecilia
• Steiniche, Torben
• Liu, Ying
• Li, Rong
• Schmidt, Mette
• Purup, Stig
• Dagnæs-Hansen, Frederik
• Schrøder, Lisbeth Dahl
• Svensson, Lars
• Petersen, Thomas Kongstad
• Callesen, Henrik
• Bolund, Lars
• Mikkelsen, Jacob Giehm

Titel

Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1

Ist Teil von

• Disease models & mechanisms, 2017-07, Vol.10 (7), p.869-880

Ort / Verlag

England: The Company of Biologists Ltd

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Psoriasis is a complex human-specific disease characterized by perturbed keratinocyte proliferation and a pro-inflammatory environment in the skin. Porcine skin architecture and immunity are very similar to that in humans, rendering the pig a suitable animal model for studying the biology and treatment of psoriasis. Expression of integrins, which is normally confined to the basal layer of the epidermis, is maintained in suprabasal keratinocytes in psoriatic skin, modulating proliferation and differentiation as well as leukocyte infiltration. Here, we generated minipigs co-expressing integrins α2 and β1 in suprabasal epidermal layers. Integrin-transgenic minipigs born into the project displayed skin phenotypes that correlated with the number of inserted transgenes. Molecular analyses were in good concordance with histological observations of psoriatic hallmarks, including hypogranulosis and T-lymphocyte infiltration. These findings mark the first creation of minipigs with a psoriasiform phenotype resembling human psoriasis and demonstrate that integrin signaling plays a key role in psoriasis pathology.