Details

Autor(en) / Beteiligte

• Head, Steven R
• Komori, H. Kiyomi
• LaMere, Sarah A
• Whisenant, Thomas
• Van Nieuwerburgh, Filip
• Salomon, Daniel R
• Ordoukhanian, Phillip

Titel

Library construction for next-generation sequencing: Overviews and challenges

Ist Teil von

• BioTechniques, 2014-02, Vol.56 (2), p.61-77

Ort / Verlag

England: Future Science Ltd

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, we examine the critical role of sequencing library quality and consider important challenges when preparing NGS libraries from DNA and RNA sources. Factors such as the quantity and physical characteristics of the RNA or DNA source material as well as the desired application (i.e., genome sequencing, targeted sequencing, RNA-seq, ChIP-seq, RIP-seq, and methylation) are addressed in the context of preparing high quality sequencing libraries. In addition, the current methods for preparing NGS libraries from single cells are also discussed.