Details

Autor(en) / Beteiligte

• Jiang, Yi-Nan
• Gao, Yizhen
• Lai, Xianxin
• Li, Xinjie
• Liu, Gen
• Ding, Mingmei
• Wang, Zhiyi
• Guo, Zixiang
• Qin, Yinying
• Li, Xin
• Sun, Litao
• Wang, Zhao-Qi
• Zhou, Zhong-Wei

Titel

Microcephaly Gene Mcph1 Deficiency Induces p19ARF-Dependent Cell Cycle Arrest and Senescence

Ist Teil von

• International journal of molecular sciences, 2024-05, Vol.25 (9), p.4597

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• has been identified as the causal gene for primary microcephaly type 1, a neurodevelopmental disorder characterized by reduced brain size and delayed growth. As a multifunction protein, MCPH1 has been reported to repress the expression of TERT and interact with transcriptional regulator E2F1. However, it remains unclear whether MCPH1 regulates brain development through its transcriptional regulation function. This study showed that the knockout of in mice leads to delayed growth as early as the embryo stage E11.5. Transcriptome analysis (RNA-seq) revealed that the deletion of resulted in changes in the expression levels of a limited number of genes. Although the expression of some of E2F1 targets, such as and , was affected, the differentially expressed genes (DEGs) were not significantly enriched as E2F1 target genes. Further investigations showed that primary and immortalized knockout mouse embryonic fibroblasts (MEFs) exhibited cell cycle arrest and cellular senescence phenotype. Interestingly, the upregulation of p19ARF was detected in knockout MEFs, and silencing restored the cell cycle and growth arrest to wild-type levels. Our findings suggested it is unlikely that MCPH1 regulates neurodevelopment through E2F1-mediated transcriptional regulation, and p19ARF-dependent cell cycle arrest and cellular senescence may contribute to the developmental abnormalities observed in primary microcephaly.