Details

Autor(en) / Beteiligte

• Yang, Jia-Lei
• Fan, Hong
• Fu, Fan-Fan
• Guo, Bao-Lin
• Huang, Ying
• Sun, Li
• Wang, Wen-Ting
• Xing, Jun-Ling
• Hu, Xin-Tian
• Ding, Yu-Qiang
• Zhang, Kun
• Hu, Ying-Zhou
• Wang, Ya-Zhou

Titel

Transient neurogenesis in ischemic cortex from Sox2+ astrocytes

Ist Teil von

• Neural regeneration research, 2023-07, Vol.18 (7), p.1521-1526

Ort / Verlag

India: Wolters Kluwer India Pvt. Ltd

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The adult cortex has long been regarded as non-neurogenic. Whether injury can induce neurogenesis in the adult cortex is still controversial. Here, we report that focal ischemia stimulates a transient wave of local neurogenesis. Using 5′-bromo-2′-deoxyuridine labeling, we demonstrated a rapid generation of doublecortin-positive neuroblasts that died quickly in mouse cerebral cortex following ischemia. Nestin-CreER-based cell ablation and fate mapping showed a small contribution of neuroblasts by subventricular zone neural stem cells. Using a mini-photothrombotic ischemia mouse model and retrovirus expressing green fluorescent protein labeling, we observed maturation of locally generated new neurons. Furthermore, fate tracing analyses using PDGFRα-, GFAP-, and Sox2-CreER mice showed a transient wave of neuroblast generation in mild ischemic cortex and identified that Sox2-positive astrocytes were the major neurogenic cells in adult cortex. In addition, a similar upregulation of Sox2 and appearance of neuroblasts were observed in the focal ischemic cortex of Macaca mulatta. Our findings demonstrated a transient neurogenic response of Sox2-positive astrocytes in ischemic cortex, which suggests the possibility of inducing neuronal regeneration by amplifying this intrinsic response in the future.