Genes, 2021-12, Vol.12 (12), p.1999
2021
Details
- Autor(en) / Beteiligte
- Titel
- Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences
- Ist Teil von
- Genes, 2021-12, Vol.12 (12), p.1999
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Mesenchymal stem/stromal cells or medicinal signaling cells (MSC)-based therapy holds promise as a beneficial strategy for treating knee OA (osteoarthritis), but there is no standardized protocols nor mechanistic understanding. In order to gain a better insight into the human MSC from adipose tissue applied for autologous OA treatment, we performed extensive comparative immunophenotyping of the stromal vascular fraction from lipoaspirate or microfragmented lipoaspirates by polychromatic flow cytometry and investigated the cellular components considered responsible for cartilage regeneration. We found an enrichment of the regenerative cellular niche of the clinically applied microfragmented stromal vascular fraction. Sex-related differences were observed in the MSC marker expression and the ratio of the progenitor cells from fresh lipoaspirate, which, in female patients, contained a higher expression of CD90 on the three progenitor cell types including pericytes, a higher expression of CD105 and CD146 on CD31 CD34 endothelial progenitors as well as of CD73 on supra-adventitialadipose stromal cells. Some of these MSC-expression differences were present after microfragmentation and indicated a differential phenotype pattern of the applied MSC mixture in female and male patients. Our results provide a better insight into the heterogeneity of the adipose MSC subpopulations serving as OA therapeutics, with an emphasis on interesting differences between women and men.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2073-4425eISSN: 2073-4425DOI: 10.3390/genes12121999Titel-ID: cdi_doaj_primary_oai_doaj_org_article_63d3a89c9e2a4f2681ea729e03e9c22b
- Format
- –
- Schlagworte
- Adipocytes - metabolism, Adipose tissue, Adipose Tissue - metabolism, Adult, Aged, Biomarkers - metabolism, Body fat, Cartilage, Cartilage - metabolism, Cartilage diseases, CD105 antigen, CD73 antigen, Cell Differentiation - physiology, Croatia, Female, Flow cytometry, Flow Cytometry - methods, Genotype & phenotype, Humans, immunophenotyping, Immunophenotyping - methods, lipoaspirate, Male, Mesenchymal Stem Cells - immunology, Mesenchymal Stem Cells - metabolism, mesenchymal stem/stromal cells, Mesenchyme, microfragmentation, Middle Aged, Osteoarthritis, Osteoarthritis, Knee - immunology, Osteoarthritis, Knee - metabolism, Osteoarthritis, Knee - therapy, Patients, Pericytes, Phenotype, Phenotypes, Progenitor cells, Regeneration - physiology, Sex differences, Sex Factors, Stem cells, Stem Cells - metabolism, Stromal cells, Stromal Cells - metabolism, stromal vascular fraction, Stromal Vascular Fraction - immunology, Stromal Vascular Fraction - metabolism, Tissue engineering